rabbit anti cd61  (Proteintech)

Journal: International journal of molecular sciences

Article Title: Pharmacological Prevention of Ectopic Erythrophagocytosis by Cilostazol Mitigates Ferroptosis in NASH.

doi: 10.3390/ijms241612862

Figure Lengend Snippet: Figure 3. Cilostazol resolved the augmentation of PLT and RBC accumulation in NASH livers and prevented the PLT-induced increase in erythrophagocytosis in vitro. (A) H&E staining (upper) and immunohistochemistry of CD61 (lower) in liver tissue from human NASH patients. (B) Im- munohistochemistry and (C) Western blotting data of CD61 in liver tissue from the STAMTM model. * p < 0.05 and ** p < 0.01. (D) Immunohistochemistry of hemoglobin alpha in liver tissue from the STAMTM model. (E–G) Detection of CD68-FITC-labeled THP-1 cells phagocytosing PKH26-labeled RBCs. (E) Microplate fluorescence photometry. * p < 0.05 and ** p < 0.01. (F) Flow cytometry and (G) confocal microscopy. Green: CD68, Red: PKH26.

Article Snippet: The antibodies used in the above experiment are against anti-hemoglobin α (Abcam, Cambridge, MA, USA, ab92492), and CD61 (Cell signaling, Danvers, MA, USA, #13166) was performed manufacturer’s instruction.

Techniques: In Vitro, Staining, Immunohistochemistry, Western Blot, Labeling, Flow Cytometry, Confocal Microscopy